Early Phenotypical Changes Induced By Transfer of Human Fecal Microbiotafrom Colorectal Cancer Patients to Germ-Free Mice
Julien Tap1, Aurélien Amiot1,2, Véronique Jarrousse3, Sandra Guilmeau3, Jeanne Tran VanNhieu1, Philippe Langella4, Thierry Pédron5, Jean-Pierre Furet4, André Bado3, and Iradj Sobhani1,3*
Affiliation
- 1EC2M3 University Laboratory, Université Paris-Est Créteil Val de Marne UPEC, F-94000, Créteil, France
- 2Department of Gastroenterology, Henri Mondor Hospital, APHP, Creteil, France
- 3INSERM U-773, Paris 7 Denis Diderot University, F- 75890, Paris, France
- 4Commensals and Probiotics-Host Interactions, Laboratory MICALIS, Institute INRA, UMR 1319 MICALIS, F-78350, Jouy-en-Josas, France
- 5Molecular Microbial Pathogenesis Unit, INSERM U1202, Institut Pasteur, F-75724 Paris, France
Corresponding Author
Pr. Iradj Sobhani, Hopital Henri Mondor, 51 Av Mal deLattre de Tassigny 94010 Créteil-France, Phone: 33 (1) 49 81 23 58; Fax: 33 (1) 49 81 23 52, E-mail: iradj.sobhani@hmn.aphp.fr
Citation
Sobhani, I., et al. Early Phenotypical Changes Induced By Transfer of Human Fecal Microbiota from Colorectal Cancer Patients to Germ-Free Mice. (2016) Int J Cancer Oncol 4(1): 1- 11.
Copy rights
© 2016 Sobhani, I. This is an Open access article distributed under the terms of Creative Commons Attribution 4.0 International License.
Keywords
Abstract
Feces from patients with colorectal cancer (HK) and healthy individuals (HN) were inoculated orally into germ-free mice under azoxymethane (AOM) or vehicle. The fecal microbiota remained stable in germ-free (GF) mice up to 42 days after fecal transplantation. Numbers of aberrant crypt foci, levels of MATH1, and HES1 mRNAs in the colonic mucosa, were higher in HK-GF than in HN-GF mice with highest levels observed in HK-GF mice treated with AOM and significantly associated with higher Bacteroides and lower Coprococcus bacteria in stools. Thus, CRC patients’ fecal microbiota can induce early precancerous changes in the colonic mucosa in germ-free mice.
