Journal of Clinical Trials, Pathology and Case Studies
Co-variation between HERV-K RNA Expression and CD4 Cell Counts in Untreated HIV-1 Infected Individuals
- 1Department of Pathology, University of Texas Medical Branch, 301 University Boulevard, Galveston, TX, USA
- 2División de Ciencias de la Salud, Universidad de Monterrey, Nuevo León, México
- 3Internal Medicine, University of Texas Medical Branch, 301 University Boulevard, Galveston, TX, USA
- §Authors contributed equally to this work
Jianli Dong, Molecular Diagnostics Division, Department of Pathology, University of Texas Medical Branch, 301 University Boulevard, Galveston, Texas 77555-0743, USA, Tel: (409) 772-4866, E-mail: firstname.lastname@example.org
Dong, J., et al. Covariation Between HERV-K RNA Expression and CD4 Cell Counts in Untreated HIV-1 Infected Individuals. (2016) J Clin Trials Pathol Case Stud 1(1): 17- 21.
© 2016 Dong, J. This is an Open access article distributed under the terms of Creative Commons Attribution 4.0 International License.
KeywordsHERV-K expression; HIV-1 viral load; CD4 cell count; DNase I digestion
Human endogenous retroviruses (HERVs) have been implicated in the etiology of cancer, chronic inflammation, and other diseases, and emerging data supports the regulation and functional interaction between K type HERV (HERV-K) and HIV-1. In this study, we performed a reverse transcription-polymerase chain reaction (RT-PCR) assay using DNase I digestion to remove “contaminating” HERV-K genomic DNA and examined HERV-K RNA expression in plasma samples from 54 untreated, newly diagnosed, HIV-1 infected individuals. We found that, HERV-K expression did not correlate with HIV-1 viral load. However, the mean CD4 cell count was significantly lower in HERV-K RNA positive specimens. Further studies are necessary to examine the regulatory relationship between HERV-K and CD4 cell counts in HIV-1 infected individuals.