Acute Effect of Coconut Oil Consumption does not Affect Postprandial Human Cytokines in Healthy Overweight Women

The coconut oil is popularly recognized for its anti-inflammatory properties, but research has shown conflicting results. Thus, our aim was to assess the postprandial inflammatory response of moderate coconut oil intake. We evaluated the acute consumption of a breakfast, offered in a single day, containing 25 mL of coconut oil (test) or 25 mL of olive oil (control)on postprandial serum inflammatory cytokines (IL-2, IL-4, IL-6, IL-10, IL-17A, IFN-γ and TNF) in healthy overweight women. No change was observed in relation to the effect of fatty meals, the time and the interaction of both factors (meal x time). Thus, despite being a saturated fat source, coconut oil consumption did not promote an inflammatory response. Further studies are needed considering a larger intervention period to check long-term effect. *Corresponding author: Daniela Mayumi Usuda Prado Rocha, Av PH Rolfs s/n, Federal University of Vicosa, Department of Nutrition and Health, Viçosa, Minas Gerais, CEP: 36570-900, Brazil, Tel: +55 31 3899 3388; E-mail: mayumi.dani@gmail.com Received Date: October 08, 2016 Accepted Date: October 22, 2016 Published Date: October 28, 2016


Introduction
There is a great demand for weight loss strategies and reducing body fat, especially for overweight women, increasingly popularized by media, which has invested in marketing of food with "miracle" potential, and more recently highlighting the coconut oil as "miraculous" fat [1] . Coconut oil contains high proportions of Medium-Chain Fatty Acids (MCFA), mainly lauric acid (12:0) [2] . The claim for its use in obesity treatment is associated with the MCFA effects such as increasing energy expenditure and fat oxidation, also reducing waist circumference [2,3] .
Obesity is a growing global health problem aggravated by its related complications with various morbidities such as diabetes, hypertension, cardiovascular disease and cancer. According to the estimates of the World Health Organization, in 2014 more than one-third (39%) of adults in the world aged ≥ 18 years were overweight and 11% of men and 15% of women worldwide were obese [4] . Furthermore, obesity associates with a low-grade inflammatory response and is often accompanied by elevated inflammatory markers such as pro-inflammatory cytokines [5,6] .
Although the etiology of obesity is complex, diet is an important modified factor involved in obesity-induced inflammation. In addition, nutrient physiology and immunology are inextricably linked. Studies suggest that fasting or high-fat meals can induce acute episodes of nutrition-related immune activation [7,8] . In this sense, fatty acids influence many events and cellular mechanisms, playing an important role in regulating inflammatory and immune responses [9] . Study on animal model (C57BL/6J mice) demonstrated an association with coconut oil consumption with a down regulated production of pro-inflammatory cytokines (IL-6, IFN-γ) and reduced intestinal inflammation [10] . However, studies evaluating similar effect in human are scarce and controversial [11][12][13] .
www.ommegaonline.org Coconut Oil Consumption does not Affect Postprandial Human Cytokines 2 Thereby, we intend to contribute to the knowledge about the cytokines concentration, as biomarkers on inflammatory status, associated with the coconut oil consumption.
This preliminary study aimed to assess the postprandial acute effect of coconut oil intake on serum concentration of IL-2, IL-4, IL-6, IL-10, IL-17A, IFN-γ and TNF cytokines in overweight women.

Participants
Volunteers were recruited by advertising on university campus and local radio. Eligibility criteria included healthy women (aged 20 -45 years), overweight/obesity (≥ 25 kg/m²), and high body fat content (> 30%) assessed by tetra polar bioelectrical impedance method (In Body®, model Y230); weight stability (< 3 kg) and physical activity in the last 3 months.
Smokers; pregnant or lactating women; subjects with chronic and inflammatory diseases; using drugs could affect the study results; consuming caffeine ≥ 1250 mg/day and intake of one alcohol dose (10 -12g) per day were not included in the study. All participants were advised to maintain the level of physical activity and diet style throughout the duration of the study.
The study protocol was in accordance with to the Helsinki Declaration and it was approved by the Human Ethical Committee in Scientific Research (protocol number 541.836) of Universidade Federal de Viçosa, Brazil. Written informed consent was obtained from all the participants.

Study design
This pilot study was a clinical trial with a randomized, controlled, crossover design on the postprandial effect of two is ocaloric meals with fatty acids derived from coconut oil or olive oil on serum inflammatory cytokines. The study was conducted at the Laboratory of Energy Metabolism and Body Composition (LAMECC), Department of Nutrition and Health, Universidade Federal de Viçosa, Brazil. After an overnight fast of at least 12 h, 17 volunteers were recruited to reported to the laboratory and randomly consumed one of the test meals within 10 -15 min of the first (baseline) blood sampling. Blood samples were then taken sequentially at 60, 120, and 180 min after the test meal was consumed ( Figure 1). During the postprandial assessment, participants refrained from any strenuous activity and abstained from consuming any food, except water. The consumption of each test meal was separated by a washout period of at least one week between sessions.

Meal composition
The isocaloric meals (~494kcal) provided 45.7% of energy as fat, 39.7% as carbohydrate and 14.6% as proteins. The test meal main source of fat was the coconut oil, rich in medium chain saturated fatty acids. The control meal containing olive oil was rich in long-chain mono unsaturated fatty acids. The meals comprised 50 g of white bread added with 25 mL of the selected lipid source (coconut oil or olive oil), and 300 mL of water-reconstituted powdered milk (40 g) added with flavoring strawberry powder (2.7g), constituting 30 to 40% of the volunteers baseline estimated energy requirements. During storage, coconut oil and olive oil were kept refrigerated (4°C) to avoid physicochemical changes. Nutritional composition of the meals was determined according to manufacturer's information and the total caloric value was obtained from the sum of the calories corresponding to macronutrients and was expressed in kilocalories (kcal) ( Table 1).

Blood samples
Blood samples were extracted after 12 h of fast, through intravenous puncture in the median a tecubital vein using a vacuum system, with subsequent centrifugation at 3500 rpm for 15 min (4°C). Serum samples were stored at -80ºC until analysis.
Serum glucose, triglycerides and total cholesterol concentrations were determined by colorimetric enzymatic tests (Cobas Mira Plus, Roche Diagnostic Systems). The insulin concentrations were measured by electrochemilumine scence immunoassay (Elecsys-Modular-E-170, Roche Diagnostics Systems). HDL-cholesterol (HDL-c) concentrations were determined in the supernatant of serum samples following precipitation with phosphotungstic acid/Mg2+.LDL-cholesterol (LDL-c) was calculated using the Friedewald formula.

Statistical analysis
The cytokines area under the curve (AUC) and incremental area under the curve (iAUC) was calculated for cytokines from 0 to 3 hours, using the trapezoid rule in Graph Pad Prism 5 software (Graph Pad, La Jolla, CA, USA). Shapiro-Wilk's normality test was used to check for the normal distribution of data. Data are expressed as mean ± SEM or median (p25-p75 th percentiles values), when appropriate. Differences in serum cytokine concentrations of the two isoenergetic meals between time points for each test meal, and differences in cytokines concentrations between meals (coconut -olive oil) in each time point were assessed with Friedman's, followed by pair wise comparisons with a Bonferroni correction for multiple comparisons. Changes Δ (final -baseline) in cytokines concentrations and differences in the cytokines AUC and iAUC between the two isoenergetic meals were analyzed Wilcoxon signed-rank test. Statistical analysis was conducted using SPSS 20 for Windows (SPSS, Inc., Chicago, IL, USA). The criterion for statistical significance was α ≤ 0.05 for all data analyses.

Results
Seventeen women were randomly assigned to the trial, mean age at baseline of 26.69 ± 1.12 years, 27.50 ± 0.32 kg/m 2 and 37.44 ± 0.54 % body fat ( Table 2). Due to personal reasons, 15 women completed test (coconut oil) and 16 control (olive oil) interventions. A total of 14 participants completed both interventions. Data from all participants that completed the study were included in baseline analyses as well as in the baseline postprandial analyses of inflammatory biomarkers. Measurements of Th1, Th2 and Th17 serum cytokines (Tables 3 and 4) and delta cytokines revealed no significant differences in relation to the effect of the coconut or olive oil meals, between time points for each test meal, or differences in cytokines concentrations between meals (coconut -olive oil) in each time point. Also, no differences were observed in changes Δ (final -baseline) in cytokines concentrations, cytokines AUC or iAUC between the two tested meals.

Discussion
The media highlights coconut oil as a food with positive effects on health. Women are a target group of these marketing strategies due to their increasing demand for products that promote thermogenic effects and reduce weight and waist circumference, as claimed for coconut oil [12] . Other health claims also include its antioxidant and antimicrobial and anti-inflammatory properties [14][15][16] .
In our study, the intake of 25 mL (46% of meal energy) of coconut oil or olive oil (control) did not change post prandialserum anti and pro-inflammatory cytokines. Similarly, previous study evaluating the coconut oil and olive oil consumption (20% of daily value) during 5 weeks also verified no change on plasma inflammatory cytokines (IL-1β, IL-6, IL-8, IFN-γ, TNF-α) [12] . Myhrstad et al. (2011) also did not achieve significant results testing meals containing 29 g of coconut oil on postprandial plasma pro-inflammatory cytokines (IL-6 and IL-8) or their gene expression on PBMC, at3 and 6 h after consumption of the test meals. Moreover, Kontogianni et al. (2013)assessed inflammatory markers related to olive oil intake (15mL) during 6 weeks and found no significant changes in inflammatory markers, including hs-PCR and TNF-α, confirming our results. We know that saturated fats are linked to inflammatory processes [18] . However, coconut oil does not seem to have this effect, despite being source of saturated fat. In addition, coconut oil does not affect the lipemia [2] . Thus, coconut oil could be an interesting substitute for saturated fat from animal sources, possibly related to the presence of other elements including bioactive compounds.
Nevertheless, studies indicate the beneficial effect of olive oil consumption on pro-inflammatory cytokines [19,20] . PREDIMED study (PREvención con DIeta MEDiterránea), provided weekly 1 liter of olive oil for home consumption ad libitum in the context of the Mediterranean diet. After 1-year intervention, subjects with high cardiometabolic risk showed significant reduction in plasma concentrations of IL-6 and TNF receptors (TNFR60 and TNFR80) [19] . Also, the acute consumption of extra virgin olive oil (50 mL) reduced the postprandial concentration of TNF-α after 3 h, showing an anti-inflammatory effect associated with its use [20] .
First, we emphasize that the amount used in the studies cited are far superior to that used in our study, suggesting a possible anti-inflammatory dose-response relationship regard to olive oil consumption. However, we cannot state that higher doses of coconut oil would also promote similar effect. In fact, Pietraszek et al. (2011) in a postprandial acute study found an induced pro-inflammatory response for several inflammatory genes in adipose and muscle tissue associated with high dose of coconut oil (80g) consumed by healthy subjects or at risk for diabetes. In addition, in this intervention, pro-inflammatory cytokine IL-6 plasma concentrations increased in response to coconut oil consumption. Furthermore, quantities greater than applied in our research does not reflect the usual fat intake of the population studied by us.
The strengths of our study include its design (controlled, crossover clinical trial), good compliance with test meals, and the number of both pro and anti-inflammatory cytokines evaluated. However, the postprandial period of evaluation (3 h) may have been insufficient to detect changes in the evaluated inflammatory markers. In fact, the secretion of such proteins involves transcriptional and translational processes, which does not occur simultaneously, also, other regulatory mechanisms may be involved [21] . This way, it would be interesting to conduct long-term feeding trials using coconut oil to evaluate the effect on the concentration of inflammatory cytokines and thereby ascertain the impact of their use for the inflammatory state, and consequently on the health of consumers.

Conclusion
Despite being a saturated fat source, the acute consumption of coconut oil did not alter the inflammatory response in healthy adult overweight women, suggesting that the consumption of moderate amounts of coconut oil (25 mL) does not promote the increase of postprandial inflammatory response. Further studies are needed considering a larger intervention period to check long-term effect.