A Rare Case of Metastatic Primary Peritoneal Adenocarcinoma Presenting as Breast Cancer

Copyright: © 2016 Watson GA, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. A Rare Case of Metastatic Primary Peritoneal Adenocarcinoma Presenting as Breast Cancer Watson GA*, Greally M, Murphy DJ, Doyle A, Quinn C and Walshe JM Department of Medical Oncology and Pathology, St. Vincent’s University Hospital, Elm Park, Ireland

Both the breast mass and axillary lymph nodes were biopsied, and pathological analysis revealed an invasive ductal carcinoma with a nodal metastasis, ER (Oestrogen Receptor) positive, HER2 (Human Epidermal Growth Factor Receptor 2) negative.The breast demonstrated multifocal enhancement in the inferior aspect on Magnetic Resonance Imaging (MRI) and multiple abnormal left axillary nodes were noted.A staging CT of thorax, abdomen and pelvis (TAP) revealed para-aortic lymphadenopathy at the level of the renal arteries, measuring up to 1.8 cm in short axis.A CT guided biopsy of a para-aortic lymph node was positive for metastatic disease.There were no other sites of disease reported.(Figure 2).Thus at this time, the clinical picture was Stage IV breast cancer to para-aortic lymph nodes.As local disease control in the breast was a concern, chemotherapy was administered.The patient received dose dense Adriamycin and Cyclophosphamide followed by Taxolchemotherapy, which she tolerated without complication.During chemotherapy, her breast mass reduced but was still palpable.She was then commenced on anti-hormonal therapy, the aromatase inhibitor anastrozoleaiming for ongoing disease control.Follow-up breast imaging after chemotherapy showed stromal thickening in the left breast with abnormal lymph nodes in the left axilla without a focal breast mass, and physical examination revealed resolution of the changes observed at presentation.Restaging CT scan demonstrated reduced retroperitoneal adenopathy; the index para-aortic node reduced in size from 1.8 cm to 0.9 cm (Figure 3).As her disease bulk was low, the role of mastectomy was discussed for local disease control.At that point, her pathology was reviewed by another senior breast pathologist and the possibility of a gynecological primary, rather than primary breast cancer, was raised.WT-1 testing was completed and found to be positive both in the para-aortic lymph node and breast thus suggesting a gynaecological origin.Of note, this woman had a hysterectomy with removal of ovaries 12 years previous for abnormal vaginal bleeding.This surgical specimen was reviewed in light of these findings, however no malignancy was identified.Subsequent review of her imaging from the time of diagnosis revealed very subtle thickening in the omentum, suggestive of a primary peritoneal cancer.When we presented these findings to our patient, she stated that on retrospect she had been experiencing right groin pain and abdominal discomfort which had disappeared on her cytotoxic treatment.A positron emission tomography (PET) scan demonstrated low grade FDG uptake in portocaval and proximal right external iliac regions, consistent with findings on CT imaging.Serum CA-125 at this time was elevated at 53.3 u/ml (normal < 35 u/ml).She was referred to our surgical gynecology colleagues for assessment regarding surgery, however the patient reported a very low symptom burden and thus declined surgical intervention.She remained on anastrozole for two years from the time of her initial diagnosis with a normal CA-125.
At two years, she was admitted to hospital with acute cholecystitis.A CT Abdomen also revealed the development of multiple peritoneal soft tissue nodules, features consistent with peritoneal carcinomatosis.(Figure 4).She underwent laparoscopic cholecystectomy, and pathology revealed metastatic adenocarcinoma with features consistent with a peritoneal primary present in multiple submucosal and subserosal lymphatic spaces.Repeat oestrogen receptor (ER) andWT1 testing was performed on this specimen and both were positive.(Figure 5).Four years since diagnosis this lady continues to have platinum sensitive peritoneal cancer although the intervals on "break" from therapy have shortened.She was referred for genetic testing although this is very clearly one related process, metastatic peritoneal cancer to breast.She tested BRCA mutation negative.Now four years since diagnosis, our patient remains alive with good quality of life four years since diagnosis of Stage IV primary peritoneal cancer to breast.Figure 4 and 5
There have been isolated reports of ovarian or peritoneal cancer metastasizing to the breast [1] .The first case was described in 1907 by Sitzenfrey [6] .In a study performed by Hadju and Urban, 4,051 breast cancer patients were identified and the overall incidence of primary gynecologic cancers metastatic to the breast was 0.17%, with only 0.07% of metastatic disease originating from a primary ovarian tumor [3] .The largest literature review by Baykal et al. described 37 cases [7] .Dissemination of ovarian or peritoneal cancer occurs largely via intraperitoneal implantation, but also through lymphatic and haematogenous spread [8] .The most common sites of distant metastasis are the liver and the lung, but certain factors may increase the susceptibility of metastases to the breast, including the absence of hereditary breast-ovarian cancer [5][6][7][8] .
While diagnosis may be challenging it is important to differentiate between metastatic ovarian/peritoneal cancer and primary breast tumors not only to guide therapy, but also to prevent unnecessary surgical intervention.Treatment options for localized primary breast or ovarian cancer include surgery, radiation and systemic therapy (hormonal therapy and chemotherapy).However in more advanced disease, surgical intervention is less relevant.
Metastatic lesions to the breast tend to be more super-ficial and less fixed to surrounding tissues than primary breast lesions.Recent studies have shown that 85% of patients present with a solitary tumor, while only 4% have diffuse involvement [1,6] .The most common location is the upper outer quadrant (62% of patients) [1,9] .While various imaging modalities may aid in achieving a correct diagnosis, breast metastasis from primary ovarian/ peritoneal tumors share many morphological features with primary breast cancers, and this was observed in the present case.On mammography, metastatic lesions may appear as discrete, well-circumscribed masses while primary breast cancers typically appear speculated and display architectural distortion that metastases typically do not [1,7,9] .Micro calcifications may rarely be seen in ovarian cancer metastases due to the presence of psammoma bodies [10,11] .Microscopically, both cancers display marked nuclear atypia, brisk mitotic activity and papillary architectural patterns [2,11] .
Immunohistochemistry testing with tissue-specific markers may be helpful in determining the primary site of origin when an extra mammary malignancy is suspected.Ovarian carcinoma and breast carcinoma share many immunohistochemical features (e.g., ER, PR, HER2/neu, CA125, CK7, CK20) [11] .However, other candidate markers (e.g., GCDPF-15/BRST2, WT1, PAX8, PAX2, and mammaglobin) may also be useful [11] .WT1 has been identified as a reliable marker in differentiating metastatic ovarian/ peritoneal cancer from other primary sites due to its high sensitivity and low potential for aberrant expression [11,[12][13][14] .The most common histological variant of ovarian cancer associated with metastatic disease to the breast is papillary serous Adenocarcinoma [10] .WT1 is expressed in approximately 95% of serous ovarian carcinomas [15] .
Secondary breast involvement from an ovarian/peritoneal tumor may be a harbinger of widespread dissemination.Approximately 70% of patients presenting with a metastatic breast lesion have widespread metastatic disease at the time of diagnosis and prognosis is generally poor [4] .In previous studies, survival ranged from 13 days to 3.5 years , with most patients dying within 1 year [1,5,15] .Klein et al. 2010 suggest inflammatory metastatic disease to the breast conferred a graver prognosis, with a median survival of 6 months [1] .

Conclusion
Ovarian or peritoneal metastasis to the breast is rare, and diagnosis may be difficult as metastases share many common features with primary breast cancer.Clinical presentation, imaging and immunohistochemistry may aid in reaching a correct diagnosis.In our study WT1 testing was a vital component in reaching the correct diagnosis.Accurate differentiation is necessary because treatment differs significantly for patients with ovarian/peritoneal metastasis to the breast, as compared with patients with primary breast cancer.These metastases to the breast appear to confer a poor prognosis as patients often have widespread advanced disease.Despite this, our patient is alive four years since diagnosis, perhaps due to her low volume of disease at initial presentation.Physician awareness of this clinical entity is paramount in allowing early recognition and the initiation of appropriate therapy and optimizing patient outcomes.

Figure 2 :
Figure 2: Axial abdominal CT with oral and IV contrast shows an enlarged left paraortic retroperitoneal node (arrow).

Figure 3 :
Figure 3: Partial disease response.Axial CT with oral and IV contrast shows interval reduction in size of left paraortic retroperitoneal node (arrow).