Infection Features of Acute Leukemia Patients during Induction Chemotherapy: A Retrospective Analysis of 203 Cases

Infection is a major complication of chemotherapy for Acute Leukemia (AL). Two hundred and three patients with newly diagnosed AL were recruited in this study. By analyzing infection incidence (82.8%, 168/203), infection-related mortality (7.1%, 12/168), clinical features, and risk factors, as well as the correlation with efficacy for the patients during induction chemotherapy, we aimed to understand the significance of infection in patients with them, thereby guiding clinical treatment. All of the patients, 168 cases suffered from infection; among of them, there were clear sites of infection accounted for 94.6% (159/168), and the most infection sites were lungs, gastrointestinal tract, and oral cavity. 213 of pathogens were detected, including gram-negative bacilli (39.0%, 83/213), Gram-positive cocci (34.3%, 73/213), fungi (23.5%, 50/213), and virus (3.3%, 7/213). Diagnosis/clinical diagnosis of the patients with invasive fungal disease accounted for 24.6%, and the main pathogen was Candida. Multivariate analysis of the risk factor of infection showed that was neutropenia (P < 0.001, OR = 14.37, 95% CI 2.576 116.518); however, infection did not affect the rate of CR/CRi ( χ 2 =2.564, P = 0.109). Conclusion: During the first induction therapy for newly diagnosed AL patients, the rate of infection and fungal infection was high, Lung is the most common site of infection, Gram-negative bacteria is more common, agranulocytosis increase the chance of infection, it does not affect the complete remission rate. *Corresponding author: Linhua Yang, Department of Hematology, The Second Hospital of Shanxi Medical University, Taiyuan, China, E-mail: yanglh5282@163.com Received Date: December 25, 2016 Accepted Date: February 4, 2017 Published Date: February 14, 2017


Introduction
Infection is one of the most common complications in AL, especially during induction chemotherapy [1,2] . The infection-related mortality is quite high, infection could delay chemotherapy, and increase the risk of recurrence [3] , and the mortality of febrile neutropenia reaches 10 -20% [4][5][6] . Therefore, we retrospectively analyzed the infection status of 203 cases of newly diagnosed AL patients in our hospital during induction chemotherapy, and tried to understand clinical features and risk factors of the infection, and the relationship with the efficacy during initial induction treatment for acute leukemia patients, thereby guiding clinical treatment.

Regimen for induction therapy
The "7 + 3" regimen (cytarabine × 7d and anthracycline or anthraquinone × 3d) was performed for induction therapy in patients with AML (non-APL); part of the elderly patients or hypoproliferative acute leukemia(HAL) patients received CIG regimen (cytarabine, idarubicin and recombinant human granulocyte colony -stimulating factor); APL patients were treated with retinoic acid, arsenic trioxide and/or anthracyclines; ph -ALL patients were treated with VDLP ± C regimen (vincristine, daunorubicin, L-asparaginase, and dexamethasone ± cyclophosphamide), whereas ph + ALL patients were imatinib replace L-asparaginase.

Diagnostic criteria of infection and related definition
Diagnostic criteria of infection referred to "The diagnostic criteria of nosocomial infection of Chinese Ministry of Health (trial implementation)" [7] , and diagnostic criteria of neutropenia with fever [6,8,9] and Diagnostic criteria and treatment principles of invasive fungai disease in patient with hematonosis or malignancy [10][11][12] . The axillary temperature of patients during induction chemotherapy was more than 38.0°C or over 37.7°C for one hour, detailed physical examination and microbiology, imaging and laboratory test, exclude drugs and transfusion-related fever.

Statistical analysis
SPSS 17.0 software was used for statistical analysis. Mann-Whitney test was used for comparison in groups of continuous variables. Chi-square test was used for the comparison of categorical variables between groups with risk factors for infection screened by univariate analysis, then Logistic regression was performed. All comparisons were two-tailed test, and P < 0.05 was considered statistically significant difference.

Incidence and mortality of infection
The infection rate was 82.8% (168/203) during induction chemotherapy; 88 cases were male, 80 were female; 14 cases were dead, and 12 cases was due to infection (7.1%, 12/168), 5 males and 7 females, 5 elderly and 7 younger patients; 9 cases were AML (non-APL), 3 were ALL; one case was low-risk, eight were intermediate risk, and three were high risk.  In addition, the spectrum of pathogens was different for different site; the pathogens with the three largest number of strains were analyzed ( Figure 1). 82 pathogens were detected in respiratory: 44 strains were bacillus, including 10 strains of Acinetobacter baumannii, 9 strains of maltophilia Aeromonas, 6 strains of Escherichia 6 strains of coli, Burkholderia each; 14 strains were bacteria, including 9 strains of enterococci; 24 strains were fungal, including 15 strains of albicans. 54 pathogens were detected in gastrointestinal: 40 strains were cocci, all Infection of Acute Leukemia with induction treatment Yang, L., et al. being Enterococcus; 6 strains of bacillus, 4 strains of Klebsiella pneumonia, and 2 strains of Proteus; 8 pathogens were fungal, all being Candida, including 6 strains of Candida albicans. 67 pathogens were found from blood culture: 16 strains were gram-positive cocci, including 11 strains of coagulase-negative staphylococci (all were methicillin-resistance) 4 strains of Staphylococcus aureus; 25 strains of bacillus, including 10 strains of E. coli and 4 strains of Pseudomonas aeruginosa. 19 pathogens were fungi, including 10 strains of Candida albicans. 7 pathogens were virus. By analyzing of infection with different age, it was found that the common infection site of elderly patients was lung (65.2%, 30/46), gastrointestinal tract (30.4%, 14/46), blood flow (26.1%, 12/46), whereas the common infection sites of non-elderly patients was slightly different, including lung (40.1%, 63/157), gastrointestinal tract (29.9%, 47/157), and oral cavity (22.9%, 36/157); both groups there was no significant difference in terms of detection rate of bacillus and fungal and mortality, and detection rate of cocci in non-elderly group was significantly higher than that in older group (Table 2).

Analysis of risk factor
By univariate analysis of infection factors in AL patients during induction chemotherapy, it was showed that neutropenia, neutropenia time > 7 days, AML, and co-infection before chemotherapy were risk factors of infection for AL patients (Table 3). Multivariate analysis found that neutropenia was independent risk factor of infection.   (20.4) 50 cases of patients with diagnosis/clinical diagnosis invasive fungal disease, composed of 26 males and 24 females. Univariate analysis showed that age ≥ 60 years old, neutropenia, and neutropenia time > 14 days were risk factors. Multivariate analysis showed that age ≥ 60 years old and neutropenia were risk factors of fungal infection ( Table 4).

Correlation of infection with efficacy
Comparison of 168 cases of infection with 35 patients with non-infection in sex, age, risk stratification of leukemia, and chemotherapy, resulted in comparable balance. CR /CRi rate of the two groups of patients was not significant difference (Table 5).

Discussion
In this study, our data showed incidence of infection was 82.8% in 203 patients with newly diagnosed AL during induction chemotherapy, and the infection-related mortality was 7.1%, which was the main cause of death, in line with the study by Masmoudi., et al [13,14] . Moreover, sex, age, type of leukemia, risk stratification, and multi-site infection factor were not the factors for death. Clear site of infection were accounted for 94.6% (159/168), the most common site of infection was lung, which was consistent with the study by Cannas., et al [1,2,15,16] . 213 pathogens were isolated, gram-negative bacilli still predominantly [1,2,6,13,16] , where E. coli was the most common pathogen, followed by Acinetobacter baumannii, which was in line with the studies of Chinese CHINET monitoring network of drug-resistance bacteria in 2012 [17,18] . Similarly, the high rate of detection, high rate of multi-drug resistance and extensively drug-resistance [19] , often accompanying by a mixed infection, and the common site of infection in lung should be paid attention. Gram-positive cocci is followed by pathogen, unlike other epidemiological investigation [1,2,6,13] , predominantly Enterococcus, illustrating that empirical antibiotic therapy should consider local epidemiological investigation. Although Staphylococcus aureus and coagulase-negative staphylococci were reduced, coagulase-negative staphylococci was still the most common pathogen for infection in blood stream [15,17] , but the methicillin-resistant rate was increased (100%), which was significantly higher than that in other studies [20] , probably relating to less strains of coagulase-negative staphylococcus in our study. The study also found that the infection spectrum of pathogen in different infected site was significantly different: respiratory was mainly gram-negative bacilli, predominantly drug-resistant strains; Enterococcus was mainly in the gastrointestinal tract, followed by bacillus of blood culture, coccus, fungi, and virus, suggesting that the initial empirical anti-infective therapy should consider different infection pathogen [1] .
In this study, incidence of invasive fungal disease (24.6%) was significantly higher than that in CAESAR research [21] (4.94%), and the infection was mainly caused by Candida [1,12,[21][22][23] , predominantly in lung [1,21,22] . Univariate and multivariate analysis of factor affecting fungal infection, found that age ≥ 60 years and neutropenia were the risk factors for fungal infection [24] . The virus infection rate was low, mainly with EB virus.
Occurrence and severity of infection are related to the degree of neutropenia and duration [5,25] . In this study, infection of 70% patients started with neutropenia, and the vast majority of patients were with severe neutropenia or the neutropenia time was more than 7 days. Univariate analysis of factors of infection found that neutropenia, especially severe neutropenia, neutropenia > 7 days, AML, co-infection before chemotherapy were the risk factors for infection, which was in line with the study by Chindaprasirt., et al [26,27] . Multivariate analysis showed that neutropenia was the independent risk factor for infection. Unlike Li., et al [22] study, the present study found that diabetes and the use of hormones did not increase infection. In addition, although age was not the risk factor, the feature of infection was different, the fungal infection rate of blood stream in elderly patients was higher than that in younger patients (but with a higher detection rate of coccus).
Unlike other studies [27] that infection increases hospital stay, this study found no increase in hospital stay with infection. 168 cases of infection with a median length of hospital stay was 29 days (18 -56 days); for non-infected patients, median hospital stay was 28 days (21 -42 days). The difference was not significant (P = 0.59). However, it has been demonstrated that [28][29][30] , no matter AL patient achieves CR after chemotherapy, or the patient was without clinical contraindications, and even with severe neutropenia, it could not affect the discharge. But we need more clinical validation to confirm this conclusion.
It has been found that fungal infection is a prognostic factor [31] . The analysis of correlation between infection and efficacy for AL patients during induction chemotherapy showed that the CR/CRi rate of 203 cases of AL patients was 76.8% (155/203), and infection was not the factor affecting efficacy, which was not consistent with the above conclusion.
During the first induction therapy for AL patients, the rate of hospital infection and fungal infection was high, the main cause of death during induction therapy was infection. The dead patient mostly were co-infected in pulmonary, gastrointestinal tract, and blood stream with high detection rate of drug-resistant strains of pathogens. Neutropenia was an independent risk factor for infection, but the occurrence of infection did not affect the rate of CR/CRi. Therefore, during chemotherapy with neutropenia and neutropenia > 14 days, whether patients can receive a preventive anti-infective therapy, early prevention and treatment have to be performed to reduce the infection incidence and mortality during chemotherapy, which may give more opportunity for the subsequent treatment.