Journal of Pharmacy and PharmaceuticsJournal of Pharmacy and PharmaceuticsJournal of Pharmacy and PharmaceuticsJournal of Pharmacy and Pharmaceutics2576-3091Ommega Online PublishersNew Jersey, USA11210.15436/2377-1313.14.003Research ArticleRole of Metastable and Spore Hydration to Sterilize Spores by Nitrogen Gas Plasma Exposure and DPA Analysis by HPLC Combined with UV DetectionRole of Metastable and Spore Hydration to Sterilize Spores by Nitrogen Gas Plasma Exposure and DPA Analysis by HPLC Combined with UV DetectionShintaniHideharuChuo University School of Science 1-13-27 Kasuga Bunkyo 112-0003 TokyoEditor* E-mail: shintani@mail.hinocatv.ne.jp
The authors have declared that no competing interests exist.
20142611201411JPSDD-14-RA-112/00306112014231120142014Creative Commons Attribution LicenseThis is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. nbsp emsp emsp Many papers have been published so far on gas plasma sterilization They are mostly conducted by physical researchers so microbiology and chemical aspects are significantly insufficient By joining the biologists and chemists gas plasma sterilization research was outstandingly advanced The sterilization mechanism by gas plasma was not clarified until now and from the life time and some other reasons metastables or photon can be the most appropriate candidate for sterilization Spore death is explained from the hydration of dipicolinic acid in the core By attacking metastables or photons by E hv energy to spores it produced pin hole and the interior water and surrounding water penetrate into the core to hydrate DPA DPA in the surface was collected with water and enriched with SPE solid phase extraction column SPE drain was analyzed with C-18 HPLC column and eluted with an acetonitrile water 1 4 v v pH5 and detected with 235nm From the coincidence of retention time of the studied target and that of standard substance spore surface particle can be confirmed DPA The hydration process can cause within spore so spore figures are unchanged before and after sterilization 10