Journal of Cellular Immunology and Serum BiologyJournal of Cellular Immunology and Serum BiologyJournal of Cellular Immunology and Serum BiologyJournal of Cellular Immunology and Serum Biology2471-5891Ommega Online PublishersNew Jersey, USA169510.15436/2471-5891.19.1695Research ArticleMir-21-3p and Mir-21-5p as a candidate biomarker in serum and PBMCs of patients with systemic lupus erythematosusMir-21-3p and Mir-21-5p as a candidate biomarker in serum and PBMCs of patients with systemic lupus erythematosusnaeimehtavakolinia1Department of Immunology School of Medicine Tehran University of Medical Tehran Iran 2Rheumatology Research Center Tehran University of Medical Sciences Tehran IranEditor* E-mail: n.tavakolinia@yahoo.com
The authors have declared that no competing interests exist.
20183112201841JCISB-18-RA-169524092017251220182018Creative Commons Attribution LicenseThis is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.Introduction MicroRNAs miRNAs are endogenous small RNAs known for their function in post-transcriptional gene regulation miRNA regulates the differentiation and function of immune cells and its malfunction contributes to the development of various autoimmune diseases including Systemic Lupus Erythematosus SLE Recently miR-21 was identified to regulate a variety of immune cells and play a crucial role in a plethora of biological functions and diseases including development cancer and inflammation especially correlated with the pathogenesis of autoimmune diseases such as systemic lupus erythematosus Material and Methods In this report we used real-time PCR assay to compare the relative expression of mir-21-3p and mir-21-5p in SLE patients n 30 serums and PBMCs to healthy age and gender matched controls samples n 27 Results we found that mir-21-3p level was significantly increased in both serums and PBMCs of SLE patients compare to healthy controls serum p value lt 0 05 PBMCs p value lt 0 01 But the expression level of mir-21-5p was not significantly increased in none of serums and PBMCs Conclusion These data demonstrate that SLE disease is accompanied by the increased level of mir-21-3p a subset of mir-21 and there is no relation between mir-21-5p and disease state If these tow subsets of mir-21 have functional difference is under investigation and it rsquo s an idea that mir-21 can be studied as a biomarker for autoimmune diseases such as SLE 10