International Journal of Food and Nutritional Science International Journal of Food and Nutritional Science International Journal of Food and Nutritional Science International Journal of Food and Nutritional Science 2377-0619Ommega Online PublishersNew Jersey, USA173110.15436/2377-0619.18.1731Research ArticleAnti-Proliferative and Apoptotic Effects of Polygonum cuspidatum and its Bioactive Compound, Emodin, in Colorectal Carcinoma HT-29 cellsAnti-Proliferative and Apoptotic Effects of Polygonum cuspidatum and its Bioactive Compound, Emodin, in Colorectal Carcinoma HT-29 cellsShih YingChen 1Department of Food Science and Technology Chia Nan University of Pharmacy and Science Tainan Taiwan ROC 2Department of Health and Nutrition Chia Nan University of Pharmacy and Science Tainan Taiwan ROC 3Department of Anesthesiology Chi-Mei Medical Center Tainan Taiwan ROC 4Department of Recreation and Healthcare Management Chia Nan University of Pharmacy and Science Tainan Taiwan ROC 5Department of Senior Citizen Service Management Chia Nan University of Pharmacy and Science Tainan Taiwan ROC 6Department of Health Leisure and Management Yuanpei University of Science and Technology HsinChu Taiwan ROC Editor* E-mail: shihying@mail.cnu.edu.tw
The authors have declared that no competing interests exist.
20181202201851IJFNS-17-RA-173119112017090220182018Creative Commons Attribution LicenseThis is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. nbsp emsp emsp Antiproliferative and apoptotic effects of ethyl acetate from the root of Polygonum cuspidatum EAPC and its bioactive compound emodin in colorectal adenocarcinoma HT-29 cells were investigated HPLC analysis of major compounds cell viability by MTT assay the Comet assay determination of reactive oxygen species ROS levels apoptosis assay by flow cytometry and Western blot analysis were conducted The results show significant antiproliferative effects of EAPC IC50 141 4 mu g mL and emodin isolated from EAPC IC50 73 0 mu g mL on HT-29 cells HT-29 cells which were exposed to EAPC and emodin exerted apoptosis after annexin V FITC PI staining EAPC at 200 mu g mL and emodin at 100 mu g mL increase DNA damage in HT-29 cells by 75 6 and 67 8 respectively supporting that apoptosis occurred when the cells were treated with EAPC and emodin Cells treated with EAPC and emodin generated intracellular ROS In addition emodin at tested doses of 25-100 mu g mL suppressed nuclear factor kappa B NF kappa B and enhanced activator protein 1 AP-1 which are involved in both proliferation and apoptosis events EAPC and emodin demonstrated significant anti-proliferation of HT-29 cells through their pro-oxidant activity and contribution to oxidative stress which in turn leads to cell death EPAC and emodin inhibited HT-29 cell growth via apoptosis and could be considered as potential candidates for anti-colorectal cancer treatment 10