Journal of Marine Biology and AquacultureJournal of Marine Biology and AquacultureJournal of Marine Biology and AquacultureJournal of Marine Biology and Aquaculture2381-0750Ommega Online PublishersNew Jersey, USA179910.15436/2381-0750.18.1799Research ArticleInfluence of Light Stress on the Accumulation of Xanthophylls and Lipids in Haematococcus Pluvialis CCALA 1081 Grown under Autotrophic or Mixotrophic ConditionsInfluence of Light Stress on the Accumulation of Xanthophylls and Lipids in Haematococcus Pluvialis CCALA 1081 Grown under Autotrophic or Mixotrophic ConditionsEnrico Doria 1Department of Biology and Biotechnology University of Pavia Italy2Renewable Natural Resources Center of the Semi-Arid Zone CERZOS CONICET Camino La Carrindanga Km 7 8000 Bah iacute a Blanca Argentina 3National University of the South Department of Biology Biochemistry and Pharmacy San Juan 670 8000 Bah iacute a Blanca ArgentinaEditor* E-mail: enrico.doria@unipv.it
The authors have declared that no competing interests exist.
2018040420184119022018300320182018Creative Commons Attribution LicenseThis is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. nbsp nbsp nbsp The influence of light stress and trophic environmental conditions on the production of astaxanthin other xanthophylls and lipids from an Argentinian strain of Haematococcus pluvialis was investigated Microalgae cultures were incubated for two weeks in autotrophic or mixotrophic conditions with sodium acetate in the culture medium and subjected to two different light stresses HPLC analyses showed that 1 regardless of the growth conditions microalgal cells accumulated most of the astaxanthin about 90 in esterified form 2 maximal increase of astaxanthin level was observed in the culture grown in autotrophic conditions subjected to moderate light stress 90 mu mol photons m-2 s-1 while the same light regime in mixotrophic conditions led to a lower increase only 25 8 fold 3 in the case of high light stress 350 mu mol photons m-2 s-1 the adaptive response of microalgae also led to a significant increase in the amount of astaxanthin both in autotrophic 22 fold increase and in mixotrophic 16 fold increase conditions 4 an inverse correlation R2 0 977 exists between the amount of lipids and that of astaxanthin accumulated by Haematococcus grown under different growth conditions 5 lutein was found to be the most accumulated pigment in microalgae not subjected to light stress 10