Journal of Analytical, Bioanalytical and Separation TechniquesJournal of Analytical, Bioanalytical and Separation TechniquesJournal of Analytical, Bioanalytical and Separation TechniquesJournal of Analytical, Bioanalytical and Separation Techniques2476-1869Ommega Online PublishersNew Jersey, USA195110.15436/2476-1869.18.1951Research ArticleValidation of an HPLC Method Devised for the Quantitative Determination of Ropivacaine in Drug-Delivery SystemsValidation of an HPLC Method Devised for the Quantitative Determination of Ropivacaine in Drug-Delivery SystemsEneidade Paula1 Department of Biochemistry and Tissue Biology Institute of Biology University of Campinas Unicamp Campinas SP Brazil2 Department of Physiological Sciences Piracicaba Dental School Unicamp Piracicaba SP Brazil3 Department of Chemistry Federal Technological University of Paran aacute Londrina PR BrazilEditor* E-mail: eneidapaula@gmail.com
The authors have declared that no competing interests exist.
20182510201831JABST-18-RA-195125082018181020182018Creative Commons Attribution LicenseThis is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. nbsp emsp emsp Ropivacaine RVC is a long acting local anesthetic synthesized in the S enantiomeric form This work describes the development of an analytical method and validation parameters that assured acceptable characteristics suitability reliability and feasibility to quantify RVC in the presence of drug-delivery carriers such as cyclodextrins and liposomes High performance liquid chromatography HPLC was performed using a reversed-phase C18 column a mixture of acetonitrile and phosphate buffer pH 8 0 60 40 v v as the mobile phase 1 2 mL min flow rate and oven temperature of 30 deg C Ropivacaine was detected by UV absorption at 240 nm The results showed that the analytical method is accurate reproducible robust and linear over the concentration range of 0 08 ndash 1 16 mM RVC The method was applied to detect the in vitro release profile of the anesthetic loaded in two different drug-delivery systems i RVC encapsulated into egg phosphatidylcholine liposomes and ii RVC complexed with hydroxypropyl-beta-cyclodextrin HP- beta CD The release kinetics rate was significantly slower for the RVC-HP- beta CD complex than for liposomal RVC or the free drug RVC in solution To explain that diffusion ordered spectroscopy 1H-NMR DOSY experiments were conducted The results confirmed the stronger interaction of the anesthetic with HP- beta CD association constant Ka 128 M-1 than with liposomes Ka 22 M-1 in accordance with the release kinetic data In conclusion the HPLC method described was proven suitable for the quantification of ropivacaine in drug-delivery systems 10