Journal of Cellular Immunology and Serum BiologyJournal of Cellular Immunology and Serum BiologyJournal of Cellular Immunology and Serum BiologyJournal of Cellular Immunology and Serum Biology2471-5891Ommega Online PublishersNew Jersey, USA196810.15436/2471-5891.18.1946Research ArticleImmunomodulatory Effect of the Biofield Energy Treated Cell Growth Medium (DMEM) and FBS in Immune Cells (Murine Macrophage RAW 264.7)Immunomodulatory Effect of the Biofield Energy Treated Cell Growth Medium (DMEM) and FBS in Immune Cells (Murine Macrophage RAW 264.7)Snehasis Jana1Trivedi Global Inc Henderson USA2Trivedi Science Research Laboratory Pvt Ltd Bhopal IndiaEditor* E-mail: publication@trivedisrl.com
The authors have declared that no competing interests exist.
20182712201832JISB-RA-18-196819092018241220182018Creative Commons Attribution LicenseThis is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.The objective of the present study was to evaluate the immunomodulatory effect of Biofield Energy Treatment The Trivedi Effect reg on Dulbecco rsquo s Modified Eagle rsquo s Medium DMEM and fetal bovine serum FBS in murine macrophage cells RAW 264 7 The study parameters were evaluated using cell viability by MTT assay and estimation of proinflammatory cytokine like tumor necrosis factor - alpha TNF- alpha on immunomodulation using enzyme linked immune sorbent assay ELISA The cell viability using MTT assay data showed that more than 80 cell viability was observed in all the tested groups compared to the baseline control group G1 The characteristics of cell morphology in the Biofield Energy Treated DMEM without FBS G6 group showed some changes in the cell morphology as evidenced a transition from spherical to elongate and also showed maturation compared to the G1 group The level of TNF- alpha expression was significantly reduced by 23 15 in the G3 group compared to the G1 group Moreover the secretion of TNF- alpha was altered by 38 06 35 77 and 282 49 in the G4 G5 and G6 groups respectively compared to the G1 group The overall results demonstrated that the Trivedi Effect reg - Consciousness Energy Healing Treatment has an impact on DMEM and FBS by alteration of cell morphology and reducing the level of TNF- alpha expression in immune cell RAW 264 7 Therefore the Trivedi Effect reg treated DMEM and FBS might be useful as an immunomodulator for various immune-related disorders like Graves rsquo disease rheumatoid arthritis multiple sclerosis etc 10