Journal of Veterinary Science and Animal WelfareJournal of Veterinary Science and Animal WelfareJournal of Veterinary Science and Animal WelfareJournal of Veterinary Science and Animal Welfare2690-0939Ommega Online PublishersNew Jersey, USA2452Research ArticlePositive controls in the detection of genes of resistance to tetracyclines in bacteria of veterinary interestPositive controls in the detection of genes of resistance to tetracyclines in bacteria of veterinary interestNavarro OCarlosPreventive Medicine Department Faculty of Veterinary and Animal Sciences University of Chile Editor* E-mail: canavarr@uchile.cl
The authors have declared that no competing interests exist.
20192403201931JVSAW-19-RA-245212032019190320192019Creative Commons Attribution LicenseThis is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.Because the etiologic agents are mainly multi-resistant bacteria the treatment of nosocomial infections is increasingly complicated In addition because bacterial resistance is encoded by genes it becomes necessary to know and update their frequencies and to guide the control of antimicrobial resistance in hospitals Currently the Polymerase Chain Reaction PCR is the molecular tool used for the detection of these genes but positive controls are needed for the proper interpretation of their results Therefore the objective of this study was to obtain two positive controls for tetracycline resistance genes tet A and tet B from Pseudomonas aeruginosa and Pantoeaagglomerans two bacterial strains resistant to tetracycline These genes were detected by PCR sequenced and compared with data from GenBank reg The results obtained using the Clustal and BLAST program indicated a nucleotidic identity percentage NIP higher than 90 for tet B gene meanwhile lower nucleotidic identity for tet A gene is controversial Thus the presence of the tet B gene was confirmed in the studied strains and its utilization as positive controls can be suggested The obtaining of strain that may be used as positive control for tet A gene was not achieved however new primers are proposed 10