Journal of Cellular Immunology and Serum BiologyJournal of Cellular Immunology and Serum BiologyJournal of Cellular Immunology and Serum BiologyJournal of Cellular Immunology and Serum Biology2471-5891Ommega Online PublishersNew Jersey, USA77910.15436/2471-5891.16.779Research ArticleCross Species applicability of Anti-human plasma IgY affinity columnCross Species applicability of Anti-human plasma IgY affinity columnMark S.Baker1Department of Chemistry and Biomolecular Sciences Faculty of Science Macquarie University NSW 2109 Australia 2Department of Biomedical Sciences Faculty of Medicine and Health Sciences Macquarie University NSW 2109 Australia 3Wollongong Hospital South Eastern Sydney and Illawarra Area Health Service Wollongong NSW 2500 AustraliaEditor* E-mail: mark.baker@mq.edu.au
The authors have declared that no competing interests exist.
20161106201621JISB-16-RA-77910022016060620162016Creative Commons Attribution LicenseThis is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. nbsp emsp emsp In order to improve the sensitivity of proteomic analysis our group has developed a chicken antibody based column that has successfully depleted high abundance proteins HAPs from human plasma prior to mass spectrometry analysis The use of chicken as a host for polyclonal antibodies production conveys many advantages including but not limited to the capabilities of producing large quantities of IgY antibodies with minimal antigen quantities and enhanced immunogenicity exhibited by their immune systems against foreign immunogens In this study we explored the ability to use an anti-human IgY affinity column with a variety of plasma samples derived from different animals and demonstrate this column rsquo s ability to deplete the two most common HAPs albumin and immunoglobulins reproducibly in phylogenetically related species Hence our study highly recommends that IgY-based immune depletion technologies could be used to capture targeted proteins among phylogenetically related species without having to produce separate columns for each clade 10