Journal of Bioinformatics, Proteomics and Imaging AnalysisJournal of Bioinformatics, Proteomics and Imaging AnalysisJournal of Bioinformatics, Proteomics and Imaging AnalysisJournal of Bioinformatics, Proteomics and Imaging Analysis2381-0793Ommega Online PublishersNew Jersey, USA97410.15436/2381-0793.16.974Research ArticleA Novel Imaging Analysis Method for Capturing Pharyngeal Constriction During SwallowingA Novel Imaging Analysis Method for Capturing Pharyngeal Constriction During SwallowingWilliam GPearson 1Department of Cellular Biology amp Anatomy Medical College of Georgia Augusta University Augusta GA 2Department of Otolaryngology-Head amp Neck Surgery Medical University of South Carolina Charleston SC Editor* E-mail: wpearson@augusta.edu
The authors have declared that no competing interests exist.
20162508201621JIS-16-RA-97420062016190820162016Creative Commons Attribution LicenseThis is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. emsp emsp Videofluoroscopic imaging of swallowing known as the Modified Barium Study MBS is the standard of care for assessing swallowing difficulty While the clinical purpose of this radiographic imaging is to primarily assess aspiration risk valuable biomechanical data is embedded in these studies Computational analysis of swallowing mechanics CASM is an established research methodology for assessing multiple interactions of swallowing mechanics based on coordinates mapping muscle function including hyolaryngeal movement pharyngeal shortening tongue base retraction and extension of the head and neck however coordinates characterizing pharyngeal constriction is undeveloped The aim of this study was to establish a method for locating the superior and middle pharyngeal constrictors using hard landmarks as guides on MBS videofluoroscopic imaging and to test the reliability of this new method Twenty de-identified normal MBS videos were randomly selected from a database Two raters annotated landmarks for the superior and middle pharyngeal constrictors frame-by-frame using a semi-automated MATLAB tracker tool at two time points Intraclass correlation coefficients were used to assess test-retest reliability between two raters with an ICC 0 99 or greater for all coordinates for the retest measurement MorphoJ integrated software was used to perform a discriminate function analysis to visualize how all 12 coordinates interact with each other in normal swallowing The addition of the superior and middle pharyngeal constrictor coordinates to CASM allows for a robust analysis of the multiple components of swallowing mechanics interacting with a wide range of variables in both patient specific and cohort studies derived from common use imaging data 10