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Publish Date : 2017-04-28
Journal of Medicinal Chemistry and Toxicology

Comparative Analysis of venom of two Geographically Distinct European Adders

Vladimira Tomeckova
Vladimíra Tomeckova1*, Zuzana Andrejcakova2, Vladimír Petrilla2, Vladimír Komanický3, Monika Petrillová4, Drahomíra Sopková2, Peter Korim5, Tomas Samuely3
Article information 

Affiliation

  • 1Department of Medical and Clinical Biochemistry, Pavol Jozef Šafárik University in Košice, Faculty of Medicine, Slovak Republic.
  • 2Department of Physiology, University of Veterinary Medicine and Pharmacy, Komenského 73, 041 81, Košice, Slovak Republic
  • 3Institute of Physics, Pavol Jozef Šafárik University in Košice, Faculty of Science, Slovak Republic.
  • 4Department of General Education Subjects, University of Veterinary Medicine and Pharmacy, Komenského 73, 041 81, Košice, Slovak Republic.
  • 5Department of Environment, Veterinary legislation and Economics, University of Veterinary Medicine and Pharmacy, Komenského 73, 041 81, Košice, Slovak Republic.

Corresponding Author

Vladimra Tomeckova, Department of Medical and Clinical Biochemistry, Pavol Jozef Šafárik University, Faculty of Medicine, Slovak Republic. Tr. SNP 1, 040 11 Košice, Mobile: +421907438254; E-mail: vladimira.tomeckova@upjs.sk

Citation

Tome?ková, V., et al. Comparative Analysis of Venom of Two Geographically Distinct European Adders. (2017) J Med Chem Toxicol 2(2): 71- 78.

Copy rights

© 2017 Tomečková, V. This is an Open access article distributed under the terms of Creative Commons Attribution 4.0 International License.

Keywords

Abstract

Introduction: The study compares the venom of species, Vipera berus, from two geographically distinct areas. Individuals living in Slovakia produce larger volumes of venom in a venom gland compared to individuals living in Poland.
Methods: The complex molecular structure of individual native adder venom was recorded by synchronous fluorescence fingerprint, atomic force microscopy and protein electrophoresis.
Results: Fluorescence spectroscopy revealed differences in Slovak and Polish male and female structure of venoms and showed higher fluorescence intensity in Polish adder venom compared to Slovak adder venom. The similar pattern of venoms of snakes living in distinct area was observed by atomic force microscopy. The qualitative and quantitative protein differences in both albumin-like and globulin-like regions in adder venoms were detected by gel electrophoresis.
Conclusion: Our results exhibited differences in venom molecular structure of species Vipera berus from the distinct area.