Gene Polymorphisms of Cytokines IFN γ +874 (A/T), TNF Α -308 (G/A), IL-6 -174 (G/C), L’il-10 -1082 (A/G), -819 (C/T) -592 (C/A), TGF-β1 +869 (T /C), +915 (G/C) in Algerian Type 1 Diabetes and Latent Autoimmune Diabetes in Adults (LADA)
Rachida Raache1,2, 3*, Habiba Amroun2,3, Malha Azzouz4, Esma Mihoubi1, Aissa Boudiba4, Chafia Touil-Boukoffa1, Mohamed Cherif Abbadi2,3, Nabila Attal2,3
Affiliation
- 1Laboratoire des cytokines et NO Synthases (FSB-USTHB), Université des Sciences et de la Technologie Houari Boumediene (USTHB), Alger, Algérie
- 2Département d’Immunologie, Institut Pasteur D’Algérie (IPA), Alger Algérie
- 3Laboratoire immunopathologie te immunogénétique (LIGIP) Alger Algérie
- 4Service de diabétologie CHU Mustapha, Alger, Algérie
Corresponding Author
Dr R. Raache, Laboratoire des cytokines et NO Synthases (FSB-USTHB), Université des Sciences et de la Technologie Houari, Boumediene (USTHB), Alger, Algérie, Tél : 00213554823144; Email : E-mail: raache_ipa@yahoo.fr
Citation
Raache, R., et al. Gene Polymorphisms of Cytokines IFN γ +874 (A/T), TNF Α -308 (G/A), IL-6 -174 (G/C), L’il-10 -1082 (A/G), -819 (C/T) -592 (C/A), TGF-β1 +869 (T/C), +915 (G/C) in Algerian Type 1 Diabetes and Latent Autoimmune Diabetes in Adults (LADA). (2017) J diab Obes 4(2): 1- 7.
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© 2016 Shah, S.T. This is an Open access article distributed under the terms of Creative Commons Attribution 4.0 International License.
Keywords
Abstract
Background: We aimed to investigate the association between five cytokines (IFN γ +874 (A/T), TNF Α -308 (G/A), IL-6 -174 (G/C), L’il-10 -1082 (A/G), -819 (C/T) -592 (C/A), TGF-β1 +869 (T /C), +915 (G/C) and patients with type 1 diabetes (T1D) and Latent Autoimmune Diabetes in Adults (LADA).
Methods: Polymorphisms of cytokine were determined using the Sequence Specific Primers method (PCR-SSP) in 50 patients and 74 controls.
Results: The results showed significantly higher frequency of IFN γ +874 TA genotype (65.38% vs. 45.95%, pc = 0.046) in T1D vs. controls. While T allele (56.25% vs. 35.14%) in LADA vs. controls. While significantly higher frequency of TNF γ (-308G/A) A allele (26.92% vs. 13.51%) in T1D vs. controls and significantly higher frequency of GA Genotype (41.6% vs. 24.32%) in LADA vs. controls. While the results showed significantly higher frequency of IL-10 -1082 A/G) G/A genotype (65. 38% vs. 45.95) in T1D and LADA versus control and IL-10 -592 (C/A) C allele (84% vs. 68.92%) Regarding polymorphisms of IL-6 no significant difference was observed between T1D, LADA and controls.
Conclusion: These results suggest that the secretion low of IFN-γ and high secretion of TNF- α may play an important role in protection and susceptibility to autoimmune diabetes in the Algerian population.