Journal of Analytical, Bioanalytical and Separation Techniques
Incorporation of Radiolabelled Substrates into Mycobacterial Lipids of M. Bovis BCG and M. Kansasii
Department of Biochemistry, National JALMA Institute for Leprosy and Other Mycobacterial Diseases, Tajganj, Agra, India
Krishnamurthy Venkatesan, Department of Biochemistry, National JALMA Institute for Leprosy and Other Mycobacterial Diseases, Tajganj, Agra 282004, India, Tel: 91-562-2331751/ Fax: 0562-2331755; E-mail: firstname.lastname@example.org>
Nirmala Deo., et al. Incorporation of Radiolabelled Substrates into Mycobacterial Lipids of M. Bovis BCG and M. Kansasii. (2017) J Anal Bioanal Sep Tech 2(1): 1- 5.
© 2017 Nirmala Deo. This is an Open access article distributed under the terms of Creative Commons Attribution 4.0 International License.
KeywordsPhenolic glycolipid; Pthiocerol dimycocerosate; Mycolates; Mycobacteria; Palmitic acid; Sodium-n-valerate; Sodium-n- acetate; Sodium-n- propionate
Background: Diseases caused by microorganisms of the genus Mycobacterium continue to be a serious problem. The cell envelope constitutes up to 60% of the dry weight of lipids. The cell wall of the mycobacterium is essential for their growth and survival in the infected host. The cell wall lipids have been isolated from M. bovis BCG and M. kansasii mycobacterial strains and studied the incorporation of radiolabelled substrates for exploring the potential involvement of lipid biosynthetic pathways.
Methods: We have incubated M. bovis, BCG and M. kansasii with radiolabelled
substrates- 1-C14-palmitic acid, 1-C14-sodium-n-valerate, 1-C14-sodium-n-acetate and 1-C14-sodium-n-propionate and calculated the incorporation in PGL, PDIM and mycolates by Liquid Scintillation Counter.
Results: We have found the percentage of assimilation of 1-C14-palmitic acid into lipids of M. bovis BCG and M. kansasii were PGL: 0.39%, PDIM: 0.70% & Mycolates: 0.30% and PGL: 0.42%, PDIM: 0.81% & Mycolates: 1.45% respectively. The percentage of assimilation of 1-C14-sodium-n-valerate into lipids of M. bovis BCG and M. kansasii were PGL: 0.28%, PDIM: 0.58% & Mycolates: 1.05% and PGL: 0.35%, PDIM: 0.68% & Mycolates: 0.90% respectively. The percentage of assimilation of 1-C14- sodium-n- acetate into lipids of M. bovis BCG and M. kansasii were PGL: 0.45%, PDIM: 0.80% & Mycolates: 1.45% and PGL: 0.48%, PDIM: 0.90% & Mycolates: 1.50% respectively. The percentage of assimilation of 1-C14-sodium-npropionate into lipids of M. bovis BCG & M. kansasii were PGL: 0.48%, PDIM: 0.85% & Mycolates: 1.40% and PGL: 0.50%, PDIM: 0.90% and Mycolates: 1.48% respectively.
Conclusion: The findings of this study suggest the usefulness of radiolabelled substrate incorporation approach for exploring the involvement of lipid biosynthetic pathway.