Journal of Environment and Health Science
Phenotypic Copper Resistance in Aerobic Intestinal Bacteria from Children with Different Levels of Copper-Exposure
- aDepartment of Microbiology, RamÃ³n y Cajal University Hospital, IRYCIS, Madrid, Spain
- bInstitute for Health Research, IMIB, Virgen de la Arrixaca University Hospital, Murcia, Spain
- cCentre for Research in Environmental Epidemiology (CREAL), Barcelona, Spain
- dEpidemiology and Environmental Health Joint Research Unit. FISABIO-Universitat Jaume I-Universitat de Valencia, Spain
- ePublic Health Division of Gipuzkoa, Basque Government, Spain. BIODONOSTIA Health Research Institute, Spain
- fSpanish Project for Environment and Childhood Research (INMA), Sabadell, Spain
- gSpanish Consortium for Research on Epidemiology and Public Health (CIBERESP), Carlos III Health Institute, Madrid, Spain
- hUniversitat Pompeu Fabra UPF, Barcelona, Spain
- iCenter for Genomic Regulation (CRG), the Barcelona Institute of Science and Technology, Barcelona, Spain; IMIM, Hospital del Mar Medical Research Institute, Barcelona, Spain
- #A.S.V and A.R contributed equally to this work
Fernando Baquero, Department of Microbiology, RamÃ³n y Cajal University Hospital, IRYCIS and Spanish Consortium for Research on Epidemiology and Public Health (CIBERESP), Carlos III Health Institute, Carretera de Colmenar, Km 9.100. 28034 Madrid, Spain, Tel: 34-913368330; E-mail: email@example.com
Baquero, F., et al. Copper Resistance in Aerobic Intestinal Bacteria from Children with Different Levels of Copper-Exposure. (2017) J Environ Health Sci 3(1): 1- 13.
Â© 2017 Baquero, F. This is an Open access article distributed under the terms of Creative Commons Attribution 4.0 International License.
KeywordsCopper-resistance; E. coli; K. pneumoniae; Copper-exposure; Healthy children
Copper is an environmental element, essential for life, to which humans are exposed by inhalation, hand-to-mouth contamination, or ingestion of food and water. In this work, bacterial copper susceptibility (amount of copper able to inhibit bacterial growth) of aerobic bacteria from the intestinal microbiota of healthy children in Spain was explored. To establish the possible effect of childrenâ€™s exposure to copper in the selection of copper-resistant organisms, the prevalence of copper-resistance among bacteria isolated in stool samples of 233 children belonging to the INMA cohort of the Spanish Project for Environment and Childhood Research was studied. Stool samples were seeded into Szybalsky-type agar plates containing specific culture media and a gradient of copper sulphate (<0.68-2.05 mm="