Journal of Medicinal Chemistry and Toxicology
Programmed Cell Death of Cultured A549 Lung Epithelial Cells Induced by Sodium Arsenite Exposure
- 1Department of Environmental Health Science, School of Public Health and Tropical Medicine, Tulane University, New Orleans, LA 70112, USA
- 2School of Health Sciences, University of Newcastle, Callaghan, NSW 2308, Australia
- 3Clinical Laboratory Tests Center, The Affiliated People’s Hospital of Inner Mongolia Medical University, Inner Mongolia Autonomous Region, 010010,China
- 4Benjamin Franklin High School, New Orleans, LA70116, USA
He Wang, School of Health Sciences, University of Newcastle, Callaghan, NSW2308, Australia, Tel: (02) 49217735; E-mail: firstname.lastname@example.org
Wang, H., et al. Programmed Cell Death of Cultured A549 Lung Epithelial Cells Induced by Sodium Arsenite Exposure.. (2017) J Med Chem Toxicol 2(2): 85-89.
© 2017 Wang, H. This is an Open access article distributed under the terms of Creative Commons Attribution 4.0 International License.
KeywordsSodium arsenite (NaAsO2); Lung epithelial cells; Oxidative stress; Programmed cell death
The purpose of this experiment is to test if there is a potential for human toxicity to sodium arsenite (NaAsO2) by examining cell death in A549 cells acutely exposed NaAsO2. In this experiment, A549 cells were plated according to experimental requirements and grown to 70 - 80% confluence before treatment with NaAsO2 of different concentrations. Methylthiazol Tetrazolium (MTT) Assay and Acridine Orange (AO) staining were used to visualize and assess the cytotoxic effects on cell viability. Autophagy of cells was determined by formation of autophagosomes and ratio of induction of light chain 3-II (LC3-II) and light chain 3-I (LC3-I). Western Blot was used to determine apoptosis and Real-time PCR was conducted to quantify the changes in the levels of Caspase-3 and PARP (Poly (ADP-ribose) polymerase) levels. This study demonstrated that exposure to NaAsO2 in vitro can lead to cell death. At low concentration NaAsO2 can induce autophagy and at high concentration it can induce apoptosis. The work presented in this poster shows the initial cellular response of human lung epithelial A549 cells to acute exposure to NaAsO2 in vitro.