Quantification of Free and Bound Phenolics in Bio-Waste Pomegranate Peel and Formulation of Punicalagin Rich Rice Extruded Snacks
Citation
Robin, J., et al. Quantification of Free and Bound Phenolics in Bio- Waste Pomegranate Peel and Formulation of Punicalagin Rich Rice Extruded Snacks. (2017) Int J Food Nutr Sci 4(2):98- 104.
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© 2017 Robin, J., et al. This is an Open access article distributed under the terms of Creative Commons Attribution 4.0 International License.
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Abstract
Free and bound phenolics were studied in pomegranate peel and incorporation of punicalagin in rice extruder snacks. Total phenolic content was calculated higher in pomegranate phenolic incorporated rice extruded snacks in free or soluble form as 97.62 mg GAE/g and in residual bound form as 92.56 mg GAE/g. It seems to be higher because of the total of phenolics of rice and pomegranate peel. Similarly, higher value of total flavonoids content also seen in its rice extruder snacks. In DPPH test, the IC50 value of free and bound phenolics of fresh pomegranate peel were 7.05 and 4.95 μg/mL, respectively. HPLC analysis showed the presence of punicalagin in pomegranate peel extract and was also present in extruded snacks in its free (0.01 mg/g) and bound (0.09 mg/g) forms. Other phenolics like p-coumaric acid (1.28 mg/g), gallic acid (0.08 mg/g), cinnamic acid (0.48 mg/g) and quercetin (0.06 mg/g) present in bound extracts of extruders product. The higher values explicit the utilization of pomegranate peels as a source of antioxidants in food.