International Journal of Food and Nutritional Science
Selenium-Enriched Agaricus Bisporus Mushroom Regulates Colonic Selenoprotein Expression in Rats Differently Under Thermoneutral and Hyperthermal Stress Conditions
Faculty of Veterinary and Agricultural Sciences, University of Melbourne, Parkville, Victoria, Australia
Ken Ng,Faculty of Veterinary and Agricultural Sciences, University of Melbourne, Melbourne, Victoria 3010, Australia.Tel: 61-3-903-531-41; E-Mail: email@example.com
Ng, K., et al. Selenium-Enriched Agaricus Bisporus Mushroom Regulates Colonic Selenoprotein Expression in Rats Differently Under Thermoneutral and Hyperthermal Stress Conditions. (2015) Int J Food Nutr Sci 2(2):112-121.
©2015 Ng, K. This is an Open access article distributed under the terms of Creative Commons Attribution 4.0 International License.
KeywordsSe-enriched mushroom; Agaricus bisporus; Oxidative stress; Heat stress; Organic Se; Glutathione peroxidase-1; Glutathione peroxidase-2; Thioredoxin reductase-1;Selenoprotein P; Total GPx; α-tocopherol
Rats under thermoneutral conditions and fed mushroom-Se in dietary supplementation showed a significant fold increase (P<0.05) in mRNA expression relative to basal treatment (thermoneutral, control diet) of GPx-1 (2.98±0.82), GPx-2 (1.8±1.4), TrxR-1 (1.83±0.64) and SeP (2.77±0.92). Supplementation with α-tocopherol alone affected the expression of GPx-1 (1.83±0.82) and SeP (2.21±0.92). Rats subjected to heat stress and fed the control diet showed significant fold increase (P<0.05) in mRNA expression relative to the basal treatment of GPx-1 (3.15±0.82) and SeP (1.93±0.92). The expression of GPx-2 (3.2±1.4) and SeP (4.23±0.92) in rats fed mushroom-Se and expression of TrxR-1 (4.10±0.64) and SeP (7.44±0.92) in rats fed α-tocopherol alone were higher. Total colonic cellular GPx activity increased 3.8 fold in rats under thermoneutral conditions and fed the mushroom-Se diet compared to basal treatment. Heat stress had a negative impact on the colonic GPx activity which was recovered by mushroom-Se, but not α-tocopherol.