Letters in Health & Biological Sciences
Suppressive Effects of Welsh Onion Extracts on Mucus Hyper-production in Human Airway Cells
- 1Sano Inc., 3-4-2 Oroshi-machi, Akita, 010-0061, Japan
- 2Faculty of Bioresource Sciences, Akita Prefectural University, Shimoshinjo Nakano, Akita 010-0195, Japan
- 3Akita Research Institute of Food & Brewing, 4-26 Sanuki, Arayamachi, Akita 010-1623, Japan
Akiko Takashima, Sano Inc., 3-4-2 Oroshi-machi, Akita, 010-0061, Japan, Tel: 81-18-862-6644; Fax: 81-18-862-6646; E-mail: email@example.com
Takashima, A., et al. Suppressive Effects of Welsh Onion Extracts on Mucus Hyper-Production in Human Airway Cells. (2016) Lett Health Biol Sci 1(2): 34- 36
© 2016 Takashima, A. This is an Open access article distributed under the terms of Creative Commons Attribution 4.0 International License.
KeywordsHuman Airway Cells; Welsh Onion Extracts; Mucin glycoproteins
We investigated the suppressive effects of Welsh onion (Allium fistulosum L.) on the hyper-production of mucins in NCI-H292 human lung cancer cells. Periodic acid-Schiff staining showed that the 50% (v/v) ethanol extract of Welsh onion suppressed mucus glycoprotein production in cells, whereas the water and ethanol extracts did not. A real-time RTPCR analysis demonstrated that the 50% ethanol extract attenuated the gene expression of MUC2 and MUC5AC, which are major airway mucus components, in NCI-H292 cells stimulated with lipopolysaccharide (LPS). Furthermore, dot blot hybridization revealed that the extract at 100 μg/ml attenuated the hyper-production of the MUC5AC protein in LPS-stimulated NCI-H292 cells.