The Determination of Uric Acid in Human Saliva by Liquid Chromatography with Electrochemical Detection
Kevin Honeychurch
Affiliation
Centre for Research in Analytical, Materials and Sensors Science, Department of Applied Sciences, University of the West of England, Bristol, Frenchay Campus, Coldharbour Lane, istol, UK
Corresponding Author
Kevin C. Honeychurch, Centre for Research in Analytical, Materials and Sensors Science, Department of Applied Sciences, University of the West of England, Bristol, Frenchay Campus, Coldharbour Lane, Bristol, BS16 1QY, UK, Tel: +44 (0)117 3287357; E-mail: kevin.honeychurch@uwe.ac.uk
Citation
Honeychurch, K.C. The Determination of Uric Acid in Human Saliva by Liquid Chromatography with Electrochemical Detection. (2017) J Anal Bioanal Sep Tech 2(1): 47- 51.
Copy rights
© 2017 Honeychurch, K.C. This is an Open access article distributed under the terms of Creative Commons Attribution 4.0 International License.
Keywords
Abstract
A method employing high-performance liquid chromatography with electrochemical detection (LC-ED) has been successfully developed for the determination of uric acid in human salvia. The optimal chromatographic conditions were found to comprise a mobile phase containing 5 % methanol, 95 % 50 mM trichloroacetic acid, adjusted to pH 2.7, in conjunction with a Hypersil C18 250 mm x 4.6 mm column at a flow rate of 0.6 mL min-1. Hydrodynamic voltammetric studies were undertaken to optimize the operating potentials required for electrochemical detection. It was found that an applied potential of +1.2 V was optimum for the determination of uric acid. The proposed method was evaluated by carrying out replicate uric acid determinations on spiked and unspiked human saliva samples (%CV = 8.8 %). The data suggest that the method holds promise for clinical applications.