For many decades, most of the milk compositional research has been centered on its bioactive molecules^[1]. In addition to its biochemical composition, milk contains maternal cells that are live in fresh milk, suggesting that they have specific functions in the milk-fed young^[2]. Nevertheless, very little is known about human milk cellular component, the origin, the properties and the factors influencing breast milk cells. Although, it has been over 170 years since the first observation of cells in milk, yet it is only in the last decade that research on human milk focused the existence of breast milk stem cells^[3]. At the beginning, milk cell research was based on studies of both bovine milk and on few studies on colostrums or early lactation breast, perpetuating the thought that the leukocyte is the predominant cell type in human milk. At that time, a major hurdle was the lack of knowledge about factors that influence the cellular composition of breast milk, such as the stage of lactation or the health status of the mother and infant, which may have contributed to the classification controversy between early investigators^[1]. However, recent analyses using state of the art techniques have resulted in a shift in the previously believed dogma that the dominant cell type in human milk is the immune cell^[4]. Leukocytes constitute only a small minority (≤ 2%) of the cells in mature human milk when both the mother and infant are healthy^[4]. Indeed, in contrast to the milk of other mammalian species, such as the cow, after the first 1 - 2 weeks postpartum, human milk is dominated by non-immune cells, which is what the infant receives for the majority of the breastfeeding period^[4]. This raises the question of the nature, the fate and the functions of the remaining ≥ 98% of breast milk cells. The breast is a unique organ in that it fully matures during pregnancy and lactation, when it undergoes complete remodeling of its epithelium and stroma to support the synthesis and secretion of milk and its delivery to the infant^[5]. This ability for massive proliferation and self-renewal during pregnancy suggested the presence of stem cells in mammary tissue since 1970s^[6]. Since then, several evidences demonstrated the existence of mammary stem cells (MaSCs) in murine models. MaSCs are responsible for the changes that occur in the breast during this period because are able to produce both cell types of the mammary epithelium: myoepithelial cells and luminal cells^[6]. The identity and properties of intermediate steps along breast cellular hierarchy are still poorly understood, which is particularly so far for the human breast in its fully mature state that is during lactation. This may be partly due to the scarcity of human lactating breast and the low numbers and quiescent state of MaSCs in the resting breast. Interestingly, in 2007, Cregan et al demonstrated, for the first time, the presence of cells with mammary stem/progenitor properties in human breast milk-derived cultures^[7]. This finding candidate’s breast milk as an excellent system to study the role of stem cells in normal development and carcinogenesis, since it represents a non-invasive and easily accessible source of MaSCs. During this last decade, a growing body of evidence demonstrated that cellular composition of breast milk reflects the cellular composition hierarchy of the mammary lactating epithelium, including the presence of epithelial progenitors and cells with stem cell properties ^[8]. MaSCs found in breast milk, called by Hassiotou human breast milk stem cells (hBSCs)^[3], displayed the two hallmark properties of “stemnessâ€