biography
Is a Vectorology expert and Research leader at GEG Tech / 91140 Villebon-sur-Yvette. He did his Ph.D. in Molecular Biology and Biotechnology (Paris)Subject: Development of new lentiviral vectors for specific integration via the recombinase of the phage PhiC31
Main achievements, - Project management
- Technical writing: peer-reviewed articles, patent filing, funding applications
- Student and internship supervision
Area of Interest
Development of innovative gene transfer tools
Generation of animal models
Development of advanced therapies
top publication
Comparison between several integrase-defective lentiviral vectors reveals increased integration of an HIV vector bearing a D167H mutant Dr. Muhammad Saeed , Ms. Noëlle Dufour , cynthia C Bartholomae , Miss Urszula Sieranska , Miss Malaika Knopf , Dr. Nicolas Grandchamp , Miss Héloïse Pilet , Dr. Philippe Ravassard , Miss Julie Massonneau , Dr. Françoise Pflumio , Prof. Christof von Kalle , Dr. François Lachapelle , Dr. Alexis-Pierre Bemelmans , Dr. Manfred Schmidt ,Miss Eloïse Thierry , Dr. Sylvain Thierry , Dr. Olivier Delelis. Molecular Therapy - Nucleic Acids, 9th October 2014, accepted,
- Hybrid lentivirus-phiC31-int-NLS vector allows site-specific recombination in murine and human cells but induces DNA damage Grandchamp N, Altémir D, Philippe S, Ursulet S, Pilet H, Serre MC, Lenain A, Serguera C, Mallet J, Sarkis C. PLoS One. 2014 Jun 23;9(6):e99649. doi: 10.1371/journal.pone.0099649. eCollection 2014.
- Rapid cohort generation and analysis of disease spectrum of large animal model of cone dystrophy Kostic C, Lillico SG, Crippa SV, Grandchamp N, Pilet H, Philippe S, Lu Z, King TJ, Mallet J, Sarkis C, Arsenijevic Y, Whitelaw CB. PLoS One. 2013 Aug 19;8(8):e71363. doi: 10.1371/journal.pone.0071363. eCollection 2013. Paper rewarded in the category “best experimental work” by the Swissophthaward 2014
- Alternative oxidase expression in the mouse enables bypassing cytochrome c oxidase blockade and limits mitochondrial ROS overproduction El-Khoury R, Dufour E, Rak M, Ramanantsoa N, Grandchamp N, Csaba Z, Duvillié B, Bénit P, Gallego J, Gressens P, Sarkis C, Jacobs HT, Rustin P. PLoS Genet. 013;9(1):e1003182.doi:10.1371/journal.pgen.1003182. Epub 2013 Jan 3.
- Influence of insulators on transgene expression from integrating and non-integrating lentiviral vectors Grandchamp N,Henriot D, Philippe S, Amar L, Ursulet S, Serguera C, Mallet J, Sarkis C. Genet Vaccines Ther. 2011 Jan 4;9(1):1.