Dr. Gentile (Naples, Italy) is a recognized expert in the transglutaminase research area and has a strong background in the molecular biology and biochemical aspects of the transglutaminase (TG) enzymes expression, activity and function. His work has been focused to the tTG (TG2) characterization in normal and pathological cellular events (cell growth and differentiation, adhesion, apoptosis) by molecular biology, immunological and biochemical studies.
Cloning and characterisation of the gene for human tissue transglutaminase. -Transfection of the human tissue transglutaminase cDNA in Balb-C 3T3 mouse fibroblasts: molecular and cellular characterisation of the stable transfected cell lines. -Site direct mutagenesis of the human tissue transglutaminase cDNA. -Characterisation of rat intestinal transglutaminase and studies on transglutaminase-mediated modifications of gliadins in relation to the Coeliac Disease. -Characterization of the human tissue transglutaminase as a mediator of the cell signal transduction pathway. -Studies on the possible involvement of transglutaminases in the pathogenesis of neurodegenerative diseases. -Identification and characterisation of a transglutaminase variant from rat Nervous System and human neuroblastoma cells.