Azam Bolhassani

Assistant professor


I am Azam Bolhassani, an assistant professor working in Pasteur Institute of Iran for seven years. I am studying on vaccine design against viral infections especially HIV and HPV infections. Improvement of vaccination strategies is one of most important points in our research groups such as the use of various adjuvants or delivery systems. The use of chemotherapy associated with immunotherapy is our other research in laboratory


Area of Interest

1)Molecular Immunology
2)Evaluation of immuno-stimulatory potential
3)Immunological efficacy
4)Immunodominant parasite ‎antigens
5)Humoral immune response
6)Parasite Immunology
7)Human Vaccines & Immunotherapeutics.

top publication

1. Khavari A, Bolhassani A, Alizadeh F, Bathaie SZ, Balaram P, Agi E, Vahabpour R. Chemo-immunotherapy using saffron and its ingredients followed by E7-NT (gp96) DNA vaccine generates different anti-tumor effects against tumors expressing the E7 protein of human papillomavirus. Arch Virol. 2015 Feb;160(2):499-508

2. Aghasadeghi MR, Delbaz SA, Sadat SM, Siadat SD, Ardestani MS, Rahimi P, Bolhassani A, Roudsari RV, Bahramali G, Motevalli F, Davari M, Vakily H, Salmani AS, Nobari MB. Induction of Strong and Specific Humoral and T-helper 1 Cellular Responses by HBsAg Entrapped in the Methanobrevibacter smithii Archaeosomes. Avicenna J Med Biotechnol. 2014 Oct; 6(4): 238-245

3. Bolhassani A, Muller M, Roohvand F, Motevalli F, Agi E, Shokri M, Rad MM, Hosseinzadeh S. Whole recombinant Pichia pastoris expressing HPV16 L1 antigen is superior in inducing protection against tumor growth as compared to killed transgenic Leishmania. Hum Vaccin Immunother. 2014;10(12):3499-508

4. Bolhassani A. Cancer chemoprevention by natural carotenoids as an efficient strategy. Anticancer Agents Med Chem. 2015 Mar 2

5. Hosseinzadeh S, Bolhassani A. Immunostimulant Properties of Chemical Delivery Systems in Vaccine Development. Curr Drug Deliv. 2015 Mar 9

6. Namvar A, Bolhassani A, Khairkhah N, Motevalli F. Physicochemical properties of polymers: An important system to overcome the cell barriers in gene transfection. Biopolymers. 2015 Mar 11

7. Alizadeh F, Bolhassani A, Khavari A, Bathaie SZ, Naji T, Bidgoli SA. Retinoids and their biological effects against cancer. Int Immunopharmacol. 2014

8. Bolhassani A, Khavari A, Bathaie SZ. Saffron and natural carotenoids: Biochemical activities and anti-tumor effects. Biochim Biophys Acta. 2014

9. Saljoughian N, Taheri T, Zahedifard F, Taslimi Y, Doustdari F, Bolhassani A, Doroud D, Azizi H, Heidari K, Vasei M, Namvar Asl N, Papadopoulou B, Rafati S. Development of novel prime-boost strategies based on a tri-gene fusion recombinant L. tarentolae vaccine against experimental murine visceral leishmaniasis. PLoS Negl Trop Dis. 2013

10. Hosseinzadeh S, Bolhassani A, Rafati S, Taheri T, Zahedifard F, Daemi A, Taslimi Y, Hashemi M, Memarnejadian A. A non-pathogenic live vector as an efficient delivery system in vaccine design for the prevention of HPV16 E7-overexpressing cancers. Drug Deliv. 2013

11. Hajizadeh MR, Mokarram P, Kamali Sarvestani E, Bolhassani A, Mostafavi Pour Z. Recombinant nonstructural 3 protein, rNS3, of Hepatitis C virus along with recombinant GP96 induce IL-12, TNFα and α5 integrin expression in antigen presenting cells. Hepat Mon. 2013

12. Bolhassani A, Javanzad S, Saleh T, Hashemi M, Aghasadeghi MR, Sadat SM. Polymeric nanoparticles: Potent vectors for vaccine delivery targeting cancer and infectious diseases. Hum Vaccin Immunother. 2013

13. Salehi M., Taheri T., Mohit E., Zahedifard F., Seyed N., Bolhassani A., Rafati S. Recombinant Leishmania tarentolae encoding the HPV type 16 E7 gene in tumor mice model, Immunotherapy 2012

14. Bolhassani A., Zahedifard F. Therapeutic live vaccines as a potential anti-cancer strategy, Int. J. Cancer 2012

15. Daemi A., Bolhassani A., Rafati S., Zahedifard F., Hosseinzadeh S., Doustdari F. Different domains of glycoprotein 96 influence HPV16 E7 DNA vaccine potency via electroporation mediated delivery in tumor mice model. Immunology Letters 2012

16. Bolhassani A., Rafati S. Mini-chaperone: Potential immuno-stimulators in vaccine design. Human Vaccines & Immunotherapeutics 2012

17. Daemi A., Hosseinzadeh S., Rafati S., Zahedifard F., Rajabi Bazl M., Doustdari F., Hashemi M., Agi E., Bolhassani A. HPV16 E7-CT (gp96) fusion protein: Molecular cloning, expression and purification of a recombinant 6xHis-tagged protein in E. coli. Journal of Paramedical Sciences (JPS), 2012

18. Hosseinzadeh S., Daemi A., Bolhassani A. Heat shock proteins as the efficient vehicle in cancer. Journal of Solid Tumors, 2012

19. Mohit E., Bolhassani A., Zahedifard F., Seyed N., Eslamifar A., Taghikhani M., Samimi-Rad K., Rafati S. Immunomodulatory effects of IP-10 chemokine along with PEI600-Tat delivery system in DNA vaccination against HPV infections. Mol. Immunol. 2012

20. Mohit E., Bolhassani A., Zahedifard F., Taslimi Y., Rafati S. The contribution of NT-gp96 as an adjuvant for increasing HPV16 E7-specific immunity in C57BL /6 mouse model. Scand. J. Immunol. 2012

21. Bolhassani A. Potential efficacy of cell-penetrating peptides for nucleic acid and drug delivery in cancer. BBA-Reviews on Cancer, 2011 22. Bolhassani A., Rafati S. Non-viral Delivery Systems in Gene Therapy and Vaccine Development. Full chapter for the book entitled as \\\"Gene Therapy\\\", InTech - Open Access Publisher, Published in 2011